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Quick-DNA/RNA™ Water Kit
Cat # | Name | Size | Price | Quantity |
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Highlights
- Robust Total DNA/RNA Recovery: Concentrate and purify total DNA/RNA from large volume water samples including viral, pathogen, microbial & free nucleic acids.
- Superior Inhibitor Removal: Innovative inhibitor removal technology that yields DNA/RNA from the toughest water samples including raw wastewater, sludge, etc.
- Analytic Compatibility: Eluted DNA/RNA is ready for any sensitive downstream applications including ddPCR, RT-qPCR, NGS, etc.
Documents
Product Description
Technical Specifications
Sample Sources | Water, wastewater, sewage, sludge, finished water, natural water, river water, fresh water, salt water, etc. |
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Sample Size | • Up to 1 L low biomass liquid samples • Up to 50 mL raw wastewater sample Note: A larger sample volume can be used with the appropriate centrifugal rotor and compatible centrifuge bottle. |
DNA/RNA Purity | High quality, inhibitor-free DNA/RNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream applications including NGS, qPCR, ddPCR, RT-qPCR, and RT-ddPCR. |
Yield | Up to 25 µg DNA or 100 µg RNA eluted into ≥ 50 µl |
DNA/RNA Storage | DNA and/or RNA is eluted with DNase/RNase-Free Water and can be stored at ≤ -70°C. The addition of RNase inhibitors is highly recommended for prolonged storage. |
Equipment Needed (user provided) | Microcentrifuge, vortex, floor model centrifuge capable of spinning 50 ml conical tubes. |
Resources
Q1: Can I use this kit with filters or other concentrated water samples?
Yes. This kit can be used to extract DNA/RNA from water filters, molecular cut off filters, wastewater solids, sludge, and other concentrated water sample sources. See Section I: Sample Preparation of the instruction manual for specific processing instructions.
Q2: What equipment is required to perform this protocol?
Microcentrifuge, vortex, and floor model centrifuge capable of spinning 50 ml conical tubes.
Q3: Can I perform a DNase treatment to recover only RNA?
Yes. An optional DNase treatment can be performed during Section III: DNA/RNA Purification. Detailed instructions are provided in the appendices of the instruction manual.
Cat # | Name | Size | Price | |
---|---|---|---|---|
R1100-50 | DNA/RNA Shield | 50 ml | €73,40 | |
R1100-250 | DNA/RNA Shield | 250 ml | €260,20 | |
D7020-1-25 | Viral DNA/RNA Buffer | 25 ml | €70,10 | |
D7020-1-100 | Viral DNA/RNA Buffer | 100 ml | €193,60 | |
D7010-1-10 | DNA/RNA Binding Buffer | 10 ml | €21,40 | |
D7010-1-25 | DNA/RNA Binding Buffer | 25 ml | €58,30 | |
D7010-1-50 | DNA/RNA Binding Buffer | 50 ml | €89,20 | |
D7010-2-10 | DNA/RNA Prep Buffer | 10 ml | €21,40 | |
D7010-2-25 | DNA/RNA Prep Buffer | 25 ml | €47,60 | |
D7010-2-50 | DNA/RNA Prep Buffer | 50 ml | €74,90 | |
D7010-2-200 | DNA/RNA Prep Buffer | 200 ml | €231,60 | |
D7010-3-6 | DNA/RNA Wash Buffer (Concentrate) | 6 ml | €21,40 | |
D7010-3-12 | DNA/RNA Wash Buffer (Concentrate) | 12 ml | €35,70 | |
D7010-3-24 | DNA/RNA Wash Buffer (Concentrate) | 24 ml | €71,30 | |
W1001-1 | DNase/RNase-Free Water | 1 ml | €11,90 | |
W1001-4 | DNase/RNase-Free Water | 4 ml | €14,30 | |
W1001-6 | DNase/RNase-Free Water | 6 ml | €17,90 | |
W1001-10 | DNase/RNase-Free Water | 10 ml | €22,50 | |
C1006-50-G | Zymo-Spin IIICG Columns | 50 Pack | €65,40 | |
C1006-250-G | Zymo-Spin IIICG Columns | 250 Pack | €281,50 | |
E1010 | DNase I Set | 250 U | €75,50 | |
E1011 | DNase I Set | 1500 U | €158,30 | |
E1012 | DNase I Set | 5 x 1500 U | €710,20 | |
C1001-50 | Collection Tubes | 50 Pack | €17,90 | |
C1001-500 | Collection Tubes | 500 Pack | €61,80 | |
C1001-1000 | Collection Tubes | 1000 Pack | €107,00 | |
S6012-50 | ZR BashingBead Lysis Tubes (0.1 & 0.5 mm) | 50 Tubes | €119,90 | |
C1010-50 | Zymo-Spin IV-IR HRC Filters | 50 pack | €124,60 | |
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