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Format
Quick-DNA/RNA Viral Kit
Highlights
- Quick recovery of viral DNA/RNA from plasma, serum and other samples.
- Omits the use of organic denaturants and proteases.
- High-quality viral DNA/RNA is ready for RT-PCR, sequencing, etc.
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| C1102-25 | Zymo-Spin IIC-XL Columns | 25 Pack | €42,10 | |
| C1102-50 | Zymo-Spin IIC-XL Columns | 50 Pack | €81,50 | |
| D7020-1-25 | Viral DNA/RNA Buffer | 25 ml | €70,10 | |
| D7020-1-100 | Viral DNA/RNA Buffer | 100 ml | €193,60 | |
| R1034-2-6 | Viral Wash Buffer (concentrate) | 6 ml | €21,40 | |
| C1001-50 | Collection Tubes | 50 Pack | €17,90 | |
| R1034-2-24 | Viral Wash Buffer (concentrate) | 24 ml | €71,30 | |
| W1001-6 | DNase/RNase-Free Water | 6 ml | €17,90 | |
| W1001-1 | DNase/RNase-Free Water | 1 ml | €11,90 | |
| R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | €260,20 | |
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge |
|---|---|
| Purity | High-quality nucleic acids are ready for Next-Gen Sequencing, RT/qPCR, hybridization, etc |
| Sample Source | Plasma, serum, saliva, urine, blood, cell culture media, cellular suspensions, biopsies, swab, and fecal samples |
| Size Range | 50 nt to ~200 kb |
| Yield | 25 µg DNA and 50 µg RNA |
Resources
Documents
FAQ
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Yes, these samples are compatible.
Yes, this kit will co-purify some DNA.
Most downstream application methods for viral detection do not require DNase treatment during purification. If necessary, DNase treatment can be perform post-purification and additional components can be purchased separately (i.e., DNase I, DNA Digestion Buffer, RNA Prep Buffer and RNA Wash Buffer).
Yes, the change from clear to yellow is a result of oxidation and will not affect the performance of the buffer.
The Viral RNA Buffer is guaranteed for one year from the date of purchase even with the addition of beta-mercaptoethanol. Just be sure to close the bottle cap tightly to prevent evaporation.
In most cases, it could be because the samples contain high amounts of protein or cellular debris. To help prevent this in future preps, it’s best to add beta-mercaptoethanol to the Viral RNA buffer, and/or implement a Proteinase K digestion step.
For optimal results and detection of viral target, collect sample in DNA/RNA Shield and perform Proteinase K treatment. In addition, add beta-mercaptoethanol to the Viral RNA Buffer prior to purification, as well as perform all steps at room temperature and centrifugation speeds at 10,000-16,000 x g to ensure no buffer retention.
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