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    DNA Degradase


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    Cat # Name Size Price Quantity
    E2016 DNA Degradase 500 U €142,60
    - +
    E2017 DNA Degradase 2000 U €456,10
    - +

    Highlights

    • Quick and simple procedure for completely degrading DNA into its individual nucleotide component for quantitative analysis (e.g., whole-genome methylation analysis by HPLC, TLC, etc.).
    • 1 hour, single-enzyme digest vs. conventional 6 - 16-hour multi-step enzyme digestion protocols
    Description & Documents Specifications FAQ

    Documents

    Product Description

    DNA Degradase from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleotide components. DNA Degradase is ideal for whole-genome DNA methylation analysis by many downstream applications (i.e., HPLC, TLC, etc.). Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.

    Documents

    Technical Specifications

    Assay Condition DNA Degradase in 1X DNA Degradase Reaction Buffer. Incubate reaction mixtures at 37C for ≥ 1 hour.
    Concentration 10 U/µl
    Enzyme Inactivation 70C for 20 minutes
    Storage Store at -20C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70C.
    Unit Definition One unit (U) is the amount of enzyme required to degrade 1 µg of λ

    Resources

    The preferred substrate for Degradase and Degradase Plus is double stranded DNA. There will be only minor degradation of single stranded DNA template and no degradation of RNA template.

    Yes, you can scale the reaction up or down as necessary. For the best results, digest no more than 1 µg of DNA with 5 U (1 µl) of enzyme in 25 µl reaction volume. The reaction volume is just as important as the amount of DNA, and we recommend scaling up the volume of enzyme accordingly. For example, if the reaction volume is 100 µl, use 4 µl of DNA Degradase (Plus).

    Yes, the protocol time is a suggestion for sufficient digestion. Additional incubation time can be added to completely ensure that all sample has been digested. There is no harm in letting the reaction proceed longer.

    Yes, you can add excess enzyme to ensure full digestion.

    The best way to confirm degradation is to run the sample on a gel. Nothing should be visible for the Degradase-treated samples. You do not need to purify the reaction.

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