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Read The Zymo Research PromiseCpG Methylase (M. Sssl)
| Cat # | Name | Size | Price | Quantity |
|---|
Highlights
- For complete, in vitro methylation of DNA for methylation analysis.
- Methylation of chromatin DNA for DNA accessibility studies.
- Inhibition of endonucleases with overlapping CpG sequence recognition and [3H]-labeling of DNA.
Documents
Product Description
Technical Specifications
| Additional Information | Enzyme Commission Number (EC 2.1.1.37) |
|---|---|
| Assay Condition | CpG Methylase in 1X CpG Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 30C (Performing the Methylase reaction at 30°C rather than 37°C promotes optimal reaction kinetics). |
| Heat Inactivation | 65°C for 20 minutes |
| Source | Recombinant methylase is isolated from E. coli expressing the methyltransferase gene from Spiroplasma sp. strain MQ1. |
| Storage | Store at -20°C for up to 12 months. Avoid repeated freeze/thaw cycles of reagents. Prolonged storage is at ≤ -70°C. |
| Unit Definition | One unit (U) is the amount of enzyme required to protect 1µg of λ DNA from cleavage by BstUI restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C. |
Resources
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| E2010-2 | 10X CpG Reaction Buffer | 1 ml | €15,20 | |
| E2010-3 | 20X SAM (S-adenosylmethionine) | 200µl | €29,00 | |
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