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Read The Zymo Research PromiseLoad N’ Go ZymoBIOMICS DNA MagBead Kit
Cat # | Name | Size | Price | Quantity |
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Highlights
- Save Time and Focus on Discovery: Pre-filled 96-well reagent plate technology that offers multi-platform compatibility and reduces hands-on time by 75%.
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS™ Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
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Product Description
Technical Specifications
Bead Beating System | The innovative ZymoBIOMICS lysis system enables complete homogenization/disruption of the microbial cells walls and accurate microbial DNA analysis, free of bias. To ensure unbiased lysis, calibration of each bead-beating device is recommended by using the ZymoBIOMICS Microbial Community Standard. |
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Bioburden | A single preparation is guaranteed to contain less than 3 bacterial genomic copies per µl of eluate as determined by quantitative amplification of the 16S rRNA gene when eluted using 100 µl water. |
DNA Integrity | On average, post bead beating, genomic DNA is between 15-20 kb depending on the initial quality of the sample, making it amenable to Next-Generation Sequencing platforms requiring high molecular weight DNA. For optimal DNA integrity, collect samples in DNA/RNA Shield. |
DNA Purity | High quality, inhibitor-free DNA is eluted with ZymoBIOMICS DNase/RNase Free Water and is suitable for all downstream applications including PCR and Next-Generation Sequencing. |
DNA Recovery | Up to 5 µg total DNA. |
Sample Sources | Bacterial (including endospores), fungal, protozoan, algal, viral, mitochondrial, and host DNA is efficiently isolated from ≤50 mg of mammalian feces, ≤100 mg soil, and 5 – 20 mg (wet weight) of bacterial/fungal cells, biofilms, and water. |
Resources
Q1: What types of samples can be processed with the Load N' Go ZymoBIOMICS DNA MagBead Kit?
The Load N' Go ZymoBIOMICS DNA MagBead Kit is designed to work with a wide range of sample types, including plasma, serum, urine, cell culture media, blood, saliva, cellular suspensions, swab, fecal, and biopsy samples. Its innovative lysis system ensures efficient and unbiased nucleic acid extraction from various organisms present in these samples, making it suitable for microbial community profiling and other downstream applications.
Q2: Can the Load N' Go ZymoBIOMICS DNA MagBead Kit be used with different laboratory automation platforms, and is there a specific protocol or script needed for compatibility?
Yes, the Load N' Go ZymoBIOMICS DNA MagBead Kit is compatible with various laboratory automation platforms. Its pre-loaded plate technology is designed for seamless integration into high-throughput workflows. While the kit is generally compatible with most platforms, it is recommended to consult with the automation team from Zymo Research for appropriate scripts and scripting support. You can contact them at Automation@zymoresearch.com to ensure optimal performance and compatibility with your chosen laboratory automation system.
Cat # | Name | Size | Price | |
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C2019 | 96 Tip Combs (For V-Bottom Deep Well Plate) | 2 Combs | €14,30 | |
R1200-125 | DNA/RNA Shield (2X Concentrate) | 125 ml | €252,60 | |
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