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DNA/RNA Shield Lysis & Collection Tubes
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Documents
Product Description
Technical Specifications
| Applicable For | Next-Generation Sequencing, qPCR, microarray |
|---|---|
| Device Specs | 2 mL screwcap tube pre-filled with DNA/RNA Shield (1 mL) and ultra-high density BashingBeads (R1103 - 0.5 mm & 0.1 mm beads, R1105 - 2.0 mm beads) for homogenization. R1104 includes swab with 20 mm breakpoint. |
| Device Storage | Ambient temperature => 2 years |
| Sample Collection | 10% (v/v or w/v) |
| Sample Source | Cells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc. |
| Stability | RNA: Ambient temperature (4°C- 25°C) at least 1 month DNA: Ambient temperature (4°C- 25°C) at least 2 years DNA & RNA: Frozen (< -20°C): Indefinitely |
Resources
Q1: What sample types is DNA/RNA Shield suitable for?
DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).
Q2: Can DNA/RNA Shield be used on extracted DNA and/or RNA?
Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).
Q3: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?
RNA > 1 month
DNA > 2 years
Q4: How long can samples be stored frozen in DNA/RNA Shield?
DNA & RNA (<-20°C): Indefinitely
Q5: Can I FACS sort directly into DNA/RNA Shield?
Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 µl of FACS sorted cells into minimally 400 µl of DNA/RNA Shield).
Q6: Can I still use DNA/RNA Shield if a precipitate has formed?
This can happen on occasion due to transport or storage at lower temperatures. The reagent functionality is not affected; however, the precipitate can be resolved by heating the reagent to >37 °C.
Q7: Do I need to homogenize the sample in DNA/RNA Shield prior to storage?
No, tissues do not need to be homogenized.
Q8: How to collect solid tissues in DNA/RNA Shield?
Transfer tissues in to DNA RNA Shield at room temperature, submerge. Tissue can be homogenized or stored frozen prior to homogenization.
Q9: Is DNA/RNA ShieldTM compatible with bleach (sodium hypochlorite)?
Avoid mixing the reagent and bleach (sodium hypochlorite) or other strong oxidizing agents and acids. Clean up any accidental spills with water first.
Q10: Can the swab be submerged prior to collecting?
For any biological sample collection (nasal, throat, etc.), use a sterile swab for collection and then deposit it into DNA/RNA Shield. For application-specific questions, please reach out to technical support.
“I’m a Zymo fan! DNA/RNA Shield is a great product. It works great on my precious samples. I trust Zymo Research products and they have great tech support!!”
- Laura T. (USDA)
“We use DNA/RNA shield and it works well with adults mosquitoes, larvae, eggs. We store it at RT for weeks and at -4°C or -20°C for months, never saw any DNA degradation.”
- Umberto P. (University of Pavia)
“I did an assay using RNA/DNA shield and it works perfect. The product have excellent reproducibility when stored at 4°C for up to a month.”
- Maha AE. (University of Florida)
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