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Format
RNA Clean & Concentrator-5
Highlights
- NGS-Ready: RNA is ready for all downstream applications including Next-Gen Sequencing, RT-qPCR, hybridization, etc.
- Ultra-Pure: Eliminate contaminants and inhibitors in 5 minutes.
- Maximum Recovery: Recover >90% and elute in as little as 6 μl.
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RCC-5Sample Available
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RCC-25
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RCC-100
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96-Well
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MagBead
| Cat # | Name | Size | Price | Quantity |
|---|
| Cat # | Name | Size | Price | |
|---|---|---|---|---|
| W1001-4 | DNase/RNase-Free Water | 4 ml | €14,30 | |
| W1001-10 | DNase/RNase-Free Water | 10 ml | €22,50 | |
| C1004-50 | Zymo-Spin IC Columns | 50 Pack | €62,90 | |
| R1060-2-25 | RNA Prep Buffer | 25 ml | €47,60 | |
| R1060-2-100 | RNA Prep Buffer | 100 ml | €144,90 | |
| C1001-50 | Collection Tubes | 50 Pack | €17,90 | |
| R1003-3-12 | RNA Wash Buffer | 12 ml | €35,70 | |
| R1003-3-24 | RNA Wash Buffer | 24 ml | €70,10 | |
| R1013-2-100 | RNA Binding Buffer | 100 ml | €152,10 | |
| R1013-2-25 | RNA Binding Buffer | 25 ml | €58,30 | |
| E1010-1-4 | DNA Digestion Buffer | 4 mL | €17,90 | |
Description
Performance
Technical Specifications
| Equipment | Microcentrifuge |
|---|---|
| Format | Spin-Column |
| Purity | RNA is ready for Next-Gen sequencing, RT-PCR, microarray, hybridization, etc. A260/A280, A260/A230: > 1.8. |
| Sample Source | DNase I treated RNA, in vitro transcription products, the aqueous phase following TRIzol/chloroform or similar extraction |
| Size Range | Total RNA ≥ 17 nt |
| Yield | 10 µg RNA (binding capacity), ≥ 6 µl (elution volume) |
Resources
Documents
FAQ
Yes. The kit efficiently recovers all types of nucleic acids.
Carrier RNA can be added with no detrimental effects. The RCC is highly efficient without the need for carrier RNA, and no significant improvement in recovery has been observed with carrier RNA.
Yes, the kit efficiently removes unincorporated fluorescent dyes, radiolabeled dNTP’s and Biotin.
Product Tolerance Reference: – ≤5% Triton X-100 – ≤5% Tween-20 – ≤5% Sarkosyl – ≤0.1% SDS – ≤90% formamide – Sucrose samples should be diluted/titrated down 10- to 100- fold.
Yes, follow respective protocol for on-column DNase treatment. If the DNase does not have a protocol, proceed with in-tube DNase treatment post clean-up, then re-purify.
If the downstream application requires DNA-free RNA, we would recommend performing in-column or in-tube DNase I treatment.
Product Video
Reviews
"It isolates small RNAs in the same go, unlike MinElute columns which leaves out RNAs smaller than 200nt."
Anupma C.
"The Zymo-Spin IC column is convenient."
Lihua D.
National Institutes of Health
"Kit was easy and fast, and cleaned up my RNA samples very well. It did exactly what I needed it to do."
Caroline S.
Cornell University
Citations
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