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    Quick-DNA Fungal/Bacterial Midiprep Kit

    D6105


    Quick-DNA Fungal/Bacterial Midiprep Kit

    Cat # Name Size
    D6105 Quick-DNA Fungal/Bacterial Midiprep Kit 25 Preps
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    Highlights
    • Boost Detection: Included BashingBeads ensure complete lysis of tough-to-lyse samples.
    • Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
    • Simple: Fastest workflow (≤ 20 minutes).
    Description

    The Quick-DNA Fungal/Bacterial Midiprep Kit is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads. Zymo-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.

    Applicable For All sensitive downstream applications such as qPCR and Next-Generation Sequencing.
    Elution Volume ≥ 150 µl
    Equipment Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter
    Processing Time ≤ 20 minutes
    Processing Volume ≤ 500mg fungi or bacteria (wet weight), 5x109 bacterial cells, 5x108 yeast cells, or 5x107 mammalian cells
    Purity Typical A260/A280 & A260/A230 ≥ 1.8
    Sample Source Fungal and bacterial cell cultures, spores, pollen, nematodes, as well as other microorganisms can also be sampled.
    Size Range Capable of recovering genomic DNA up to and above ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered.
    Type Total DNA
    Yield ≤ 125 µg total DNA

    We have validated our kits with both high-speed homgenizers and low-speed disruptors. We highly recommend users to optimize their bead beating conditions for their own instruments. We recommend using a 50 ml-tube adapter to ensure that the bead beating is efficent (do not use adapters made of foam). For high-speed homogenizers, we recommend a total of 5 mins bead beating (1 min interval at 6.5 m/s with 5 mins rest, repeat 5 times). For low-speed cell disruptors, we recommend 30 mins at max speed.

    A viscous sample can indicate incomplete sample lysis. Try using less of your sample and optimize bead beating conditions (duration, speed, time) to ensure samples are thoroughly lysed. After bead beating, pellet the cell debris before moving on. Adding more Genomic Lysis buffer to the lysate can help dilute and deproteinate the sample, making the sample less viscous and more suitable for DNA recovery.

    Addition of beta-mercaptoethanol is recommended to enhance sample lysis, but can be substituted with dithiothreitol (DTT, final concentration of 10 mM). However, if bead beating is optimized and lysis is efficient, the addition of BME is not necessary and can be omitted.

    No additional RNase A treatment is required when processing samples within kit capacity. The selective chemistry allows for binding of double stranded DNA to the column and for RNA to flow through.

    Cat # Name Size Price
    D3004-1-100 Genomic Lysis Buffer 100 ml €69,30
    D3004-2-50 g-DNA Wash Buffer 50 ml €20,80
    D3004-4-16 DNA Elution Buffer 16 ml €20,80
    D3004-5-15 DNA Pre-Wash Buffer 15 ml €11,50
    C1001-50 Collection Tubes 50 Pack €17,40
    C1021-25
    S6010 ZR BashingBead Lysis/Filtration Tubes w/ 0.5 mm Beads (50 ml) 25 Pack €220,30

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